I've noticed the bolder ink on AHS/AHT and early AHU 50s.
If you feel the right side serial number, it feels like it's intaglio printed, whereas it's smooth on other notes.
I've also noticed one very odd anomaly with the AHT prefixes, it seems that the tactile dots on these notes are significantly weaker than later prefixes. I've encountered brand new bundles which barely had the dots raised, and ones from circulation where the dots have pretty much disappeared.
The AMFs I've seen recently, on the other hand have such strong tactile dots that they appear to pierce through or crack the ink on the front.