Oh yes.
I spent a 2002 $5 about a two years ago at a pizza slice place. The cashier looked at it and said it must be fake because it is missing the silver stripe. I asked him how he had lived in Canada. He looked at me and frowned. "All my life," he said. I told him that if he lived in Canada between 2002 and 2006, then he should remember that these were the ordinary issue of $5 bill during that time, before the current issue with the metallic stripe replaced it. He looked at me like I was nuts, but he eventually accepted the note.
I also spent a 1986 $2 at a different pizza slice place a few weeks ago. The cashier said "cool! Let's see if it's real." He took it to the sink and ran some water over it. Then he scratched the intaglio ink with his fingernail. "Yes, this is good," he said. "1986 it says - that's older than I am."